MOLECULAR BIOLOGY/GENOMICS Mitochondrial Gene Cytochrome b Developmental and Environmental Expression in Aedes aegypti (Diptera: Culicidae)
نویسندگان
چکیده
Cytochrome b, coded by mitochondrial DNA, is one of the cytochromes involved in electron transport in the respiratory chain of mitochondria. Cytochrome b is a critical intermediate in amitochondrial death pathway. To revealwhether cytochromebof themosquitoAedes aegypti (L.) (Diptera: Culicidae) (AeaCytB) is developmentally regulated, we used real-time quantitative polymerase chain reaction (qPCR) to examineAeaCytB gene expression levels in different developmental stages ofAe. aegypti. The qPCR showed thatAeaCytBwas expressed in each developmental stage,with peaks at Þrst and second instars andwas highly expressed in teneralmale and femaleAe. aegypti adults. Because mitochondrial genes exist as multiple copies, AeaCytB has much higher expression levels in all developmental stages in Ae. aegypti compared with nuclear genes. We also investigated the effect of abiotic environmental factors (e.g., high temperatures, ultraviolet radiation, and pesticide) on AeaCytB gene expression. Taken together, these results suggest thatAeaCytB gene plays an important role in the development of Ae. aegypti and its response to environmental stress.
منابع مشابه
Mitochondrial gene cytochrome b developmental and environmental expression in Aedes aegypti (Diptera: Culicidae).
Cytochrome b, coded by mitochondrial DNA, is one of the cytochromes involved in electron transport in the respiratory chain of mitochondria. Cytochrome b is a critical intermediate in a mitochondrial death pathway. To reveal whether cytochrome b of the mosquito Aedes aegypti (L.) (Diptera: Culicidae) (AeaCytB) is developmentally regulated, we used real-time quantitative polymerase chain reactio...
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